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α-Smooth Muscle Actin (ACTA2) Rabbit Polyclonal Antibody

Cat Number: AB-84750
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human α-Smooth Muscle Actin (ACTA2).
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry (paraffin-embedded tissues) 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Molecular Weight: 42KDa
Purification: Affinity purification
Synonyms: ACTSA
Background:

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84750-1

Western blot analysis of Mouse heart, using α-Smooth Muscle Actin (ACTA2) antibody at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-84750-2

Immunofluorescence analysis of human smooth muscle cells using α-Smooth Muscle Actin (ACTA2) Rabbit pAb
at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

AB-84750-3

Immunofluorescence analysis of mouse colon cells using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

AB-84750-4

Immunofluorescence analysis of rat rectum cells using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:100
(40x lens). Blue: DAPI for nuclear staining.

AB-84750-5

Immunohistochemistry of paraffinembedded human colon carcinoma using α-Smooth Muscle Actin Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining
protocol.

AB-84750-6

Immunohistochemistry of paraffinembedded human esophagus using α- Smooth Muscle Actin (ACTA2) Rabbit pAb
at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

AB-84750-7

Immunohistochemistry of paraffinembedded mouse lung using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

AB-84750-8

Immunohistochemistry of paraffinembedded mouse stomach using α- Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
before commencing with IHC staining protocol.

AB-84750-9

Immunohistochemistry of paraffinembedded Rat heart using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

AB-84750-10

Immunohistochemistry of paraffinembedded rat ovary using α-Smooth Muscle Actin (ACTA2) Rabbit pAb at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

References:

References for α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Product:α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Journal:Acta Pharmacologica Sinica
Application:WB
IF:6.15
Species:Homo sapiens,Mus musculus
PMID:31804606
Title:Ganoderic acid hinders renal fibrosis via suppressing the TGF-β/Smad and MAPK signaling pathways

References for α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Product:α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Journal:Pharmacological Research
Application:WB
IF:7.658
Species:Mus musculus
PMID:32151678
Title:Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis

References for α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Product:α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Journal:Oncotarget
Application:IF, IHC
IF:3.765
Species:Mus musculus
PMID:28915589
Title:Casticin Attenuates Liver Fibrosis and Hepatic Stellate Cell Activation by Blocking TGF-β/Smad Signaling Pathway.

References for α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Product:α-Smooth Muscle Actin (ACTA2) Rabbit pAb
Journal:Parasit Vectors
Application:WB
IF:3.876
Species:Mus musculus
PMID:34521449
Title:MicroRNA-497 induced by Clonorchis sinensis enhances the TGF-β/Smad signaling pathway to promote hepatic fibrosis by targeting Smad7