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α-SMA Mouse Monoclonal Antibody (1A4)

Cat Number: MABN80946
Conjugate: Unconjugated
Size: 100 μL
Concentration: 1mg/ml
Host: Mouse
Isotype: Mouse IgG1
Reactivity: Human, Mouse, Rat, Rabbit, Monkey
Applications: WB 1:500-1:2000 IHC 1:50-1:100 ICC/IF 1:200-1:1000 ELISA 1:5000-1:20000 FC 1:200-1:400
Molecular: 42kDa
Purification: Affinity purification
Synonyms: ACTA2;AAT6; ACTSA; ACTA2;α-Smooth Muscle Actin;Alpha-actin-2;Alpha actin 2
Background:

Actin alpha 2, smooth muscle, aorta, major constituent of thin filaments.

Form: Liquid
Buffer: Purified antibody in PBS with 0.05% sodium azide.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.

Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2
(3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.

Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye

Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green).
Blue: DRAQ5 fluorescent DNA dye