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Integrin Beta 1 Rabbit Polyclonal Antibody

Cat Number: AB-E9174
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human Integrin beta1. AA range:746-795
Reactivity: Human;Mouse;Rat
Applications:

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300.
Immunofluorescence: 1/200 – 1/1000.

Molecular Weight: 140kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: ITGB1; FNRB; MDF2; MSK12; Integrin beta-1;Fibronectin receptor subunit beta; VLA-4 subunit beta; CD antigen CD29
Background:

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms
have been found for this gene.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-E9174-1

Western blot analysis of lysates from Jurkat cells, using Integrin beta1 Antibody. The lane on the right is blocked with the synthesized peptide.

AB-E9174-2

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4°
overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).