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Acetylcholinesterase/ACHE

Cat Number: AB-84167
Conjugate: Unconjugated
Size: 100 ul
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide of human ACHE
Reactivity: Hu, Ms
Applications:

Western Blot: 1:200 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200
Flow Cytometry: 1:20 – 1:50

Molecular: 68kDa
Purification: Aff. Pur.
Synonyms: ACHE;ACEE;ARACHE;N-ACHE;YT
Background:

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipidcontaining structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Form: Liquid
AB-84167-1.jpg

Western blot analysis of extracts of
mouse liver, using ACHE antibody
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.

AB-84167-2.jpg

Immunohistochemistry of paraffinembedded
mouse brain using ACHE
antibody at dilution of 1:100
(40x lens).

AB-84167-3.jpg

Immunofluorescence analysis of HeLa
cells using ACHE Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

AB-84167-4.jpg

Immunofluorescence analysis of
NIH/3T3 cells using ACHE Rabbit pAb
at dilution of 1:100.
Blue: DAPI for nuclear staining.

AB-84167-5.jpg

Immunofluorescence analysis of C6 cells
using ACHE Rabbit pAb at
dilution of 1:100.
Blue: DAPI for nuclear staining.

References

Product:ACHE Rabbit pAb Journal:
Journal of Ayurveda and Integrative Medicine
Application:IHC IF: Species:
PMID:28256303
Title:Polyphenol-rich fraction of Parquetina nigrescens mitigates dichlorvos-induced neurotoxicity and apoptosis.