Actin alpha-Smooth Muscle
| Cat Number: | MABX-80133 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 200 ug |
| Clone: | 1A4 |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG2a |
| Immunogen: | N-terminal synthetic decapeptide ofα-smooth muscle actin. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blot - At 2μg/ml with the appropriate system to detect α-SMA in cells and tissues Immunohistochemistry(P) - At 0.1-0.5 μg/ml to detect α-SMA in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) - At 0.3-1.2 μg/ml to detect α-SMA in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user. |
| Purification: | Purified by the goat anti-mouse IgG affinity chromatography. |
| Background: | Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. |
| Form: | Liquid |
| Buffer: | Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative. |
| Storage: | At -20°C for one year. After reconstitution, at 4°C for three month. It can also be aliquotted and stored frozen at -20°C for longer time. |