Actin alpha-Smooth Muscle

Cat Number: MABX-80133
Conjugate: Unconjugated
Size: 200 ug
Clone: 1A4
Concentration: 1mg/ml
Host: Ms
Isotype: IgG2a
Immunogen: N-terminal synthetic decapeptide ofα-smooth muscle actin.
Reactivity: Hu, Ms, Rt

Western blot – At 2μg/ml with the appropriate system to detect α-SMA in cells and tissues
Immunohistochemistry(P) – At 0.1-0.5 μg/ml to detect α-SMA in formalin fixed and paraffin embedded tissues.
Immunohistochemistry(F) – At 0.3-1.2 μg/ml to detect α-SMA in formalin or acetone fixed tissues.
Other applications have not been tested.
Optimal dilutions should be determined by end user.

Purification: Purified by the goat anti-mouse IgG affinity chromatography.

Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

Form: Liquid
Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
Storage: At -20°C for one year. After reconstitution, at 4°C for three month. It can also be aliquotted and stored frozen at -20°C for longer time.

IHC(P) of Rat Cardiac Muscle Tissue using
Anti-alpha-Smooth Muscle Actin antibody