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Actin beta

Cat Number: MAB-24008
Conjugate: Unconjugated
Size: 100 ug
Clone: AC-015
Concentration: 1mg/ml
Host: Ms
Isotype: IgG1
Immunogen: Fusion protein of human β-actin
Reactivity: Hu, Rt, Ms
Applications:

Western Blotting 1: 10,000-1:25,000
Immunohistochemistry 1: 100 – 1:200
Immunofluorescence 1: 50: – 1 :200

Molecular: 42kDa
Purification: Aff. Pur.
Background:

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha ( ACTC1, cardiac muscle 1), alpha 1 ( ACTA1, skeletal muscle) and 2 ( ACTA2, aortic smooth muscle), beta ( ACTB), gamma 1 ( ACTG1) and 2 ( ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
MAB-24008-1.jpg

Western blot analysis of extracts of various cell lines, using β-actin antibody at 1:25,000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus Kit .ECL-2001
Exposure time: 1s

MAB-24008-2.jpg

Western blot analysis of extracts of various cell lines,
using β-actin antibody at 1:10,000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus Kit .ECL-2001
Exposure time: 30s

MAB-24008-3.jpg

Immunohistochemistry of paraffin-embedded rat spleen using β-actin antibody at dilution of 1:200 (40x lens)

MAB-24008-4.jpg

Immunohistochemistry of paraffin-embedded rat testis using β-actin antibody at dilution of 1:200