Actin beta HRP
| Cat Number: | MAB-24008HRP |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | AC.15 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein of human β-actin |
| Reactivity: | Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryzasativa |
| Applications: | Western Blot: 1:5000 – 1:50000 |
| Molecular Weight: | 43kDa |
| Purification: | Aff. Pur. |
| Background: | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using HRP-Conjugated β-actin antibody at 1:50000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 3s.