Actin beta HRP

Cat Number: MAB-24008HRP
Conjugate: Unconjugated
Size: 100 ug
Clone: AC.15
Concentration: 1mg/ml
Host: Rb
Isotype: IgG1
Immunogen: Recombinant protein of human β-actin
Reactivity: Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryzasativa

Western Blot: 1:5000 – 1:50000

Molecular Weight: 43kDa
Purification: Aff. Pur.

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of various cell lines, using HRP-Conjugated β-actin antibody at 1:50000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 3s.