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AMID Rabbit Polyclonal Antibody

Cat Number: AB-E3766
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human AIFM2. AA range:141-190.
Reactivity: Human;Mouse;Monkey
Applications:

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300.
Immunofluorescence: 1/200 – 1/1000.

Molecular Weight: 41kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: AIFM2; AMID; PRG3; Apoptosis-inducing factor 2;
Background:

Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; p53-responsive gene 3 protein Background This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-E3766-1

Western Blot analysis of various cells using AMID Polyclonal Antibody

AB-E3766-2

Western blot analysis of lysates from COS7 cells, using AIFM2 Antibody. The lane on the right is blocked with the synthesized peptide.

AB-E3766-3

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature
Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed.