Angiopoietin 2
| Cat Number: | AB-90002 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 197-496 of human Angiopoietin |
| Reactivity: | Hu, Rt, Ms |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 75kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ANGPT2;AGPT2;ANG2 |
| Background: | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using Angiopoietin
2 antibody at 1:3000
dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 5s.
Immunofluorescence analysis of HeLa
cells using Angiopoietin 2 antibody
at dilution of 1:100 (40x
lens). Blue: DAPI for nuclear staining.