Annexin VI
| Cat Number: | AB-84193 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11 |
| Reactivity: | Hu, Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 56kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ANXA11; ANX11; CAP50; annexin A11 |
| Background: | This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using ANXA11
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L)at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus .
Exposure time: 30s.
Immunofluorescence analysis of A549 cells using ANXA11 antibody.
Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody. Western blot was performed from the immunoprecipitate using ANXA11 antibody at a dilition of
1:1000.