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Anti-AMPK alpha 1 antibody (Rabbit mAb)

Cat Number: ABS150024
Conjugate: unconjugated
Size: 100 μL
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG,κ
Immunogen: Recombinant protein corresponding to Human AMPK alpha 1
Reactivity: Human, Mouse, Rat
Applications: Western Blot:1: 1000-1:2000 Immunoistochemistry (paraffin emebbed tissues) 1:500 - Immunofluorescence 1:300
Molecular: 64 kDa
Purification: Affinity purification
Synonyms: None
Background:

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Form: Liquid
Buffer: PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
WB analysis of AMPK alpha 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of AMPK alpha 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of AMPK alpha 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of AMPK alpha 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of AMPK alpha 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of AMPK alpha 1. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.