Anti-Calbindin Rabbit Monoclonal Antibody
| Cat Number: | MAB-94138 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | 4H7 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein corresponding to Mouse Calbindin |
| Reactivity: | Species, Mouse, Rat, Human, Mouse, Rat |
| Applications: | Applications Dilution WB 1: 500-1: 1000 IHC/IF 1: 1000-1: 3000 |
| Purification: | Affinity purification |
| Synonyms: | Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1, CAB27, CALB1, CALB, Calbindin 1 |
| Background: | Calbindin 1 is a protein that in humans is encoded by the CALB1 gene. The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of Calbindin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
IHC analysis of Calbindin.Sample: Human renal cancer, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , 98℃, 20 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.
IHC analysis of Calbindin.Sample: Mouse brain, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , 98℃, 20 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.