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Anti-GAD67 Rabbit Polyclonal Antibody

Cat Number: ABS111397
Conjugate: unconjugated
Size: 100 μL
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant protein corresponding to Human GAD67
Reactivity: Species, Mouse, Rat
Applications: Western Blot: 1: 500-1: 1000
Molecular: 67 kDa
Purification: Affinity purification
Synonyms: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD, GAD67
Background:

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development. It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

Form: Liquid
Buffer: PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
WB analysis of GAD67.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of GAD67.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.