Anti-Histone H3 Rabbit Polyclonal Antibody
| Cat Number: | ABS11102 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein corresponding to Mouse Histone H3.1 |
| Reactivity: | Species, Human, Mouse, Rat |
| Applications: | Western Blot: 1: 300-1: 1000 Immunohistochemistry: 1: 1000-1: 2000 Immunofluorescence: 1: 1000-1: 2000 |
| Purification: | Affinity purification |
| Synonyms: | H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o, HIST1H3A |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of Histone H3.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 300, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
WB analysis of Histone H3.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 300, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.