Anti-mTOR Rabbit Polyclonal Antibody
| Cat Number: | ABS111839 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Human mTOR |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot:1: 500-1: 1000 Immunohistochemistry(paraffin embedded tissues): 1:200-1:500 |
| Molecular: | 289 kDa |
| Purification: | Affinity purification |
| Background: | MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications. |
| Form: | Liquid |
| Buffer: | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
| Storage: | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of mTOR Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.
IHC analysis of mTOR Sample: Mouse brain 4% PFA 12-24h. Antigen retrieval: Citrate buffer , 98℃, 20 min. Primary antibody:
1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG ,
1: 200, RT, 1h.
IHC analysis of mTOR Sample: Rat brain 4% PFA 12-24h. Antigen retrieval: Citrate buffer , 98℃, 20 min. Primary antibody:
1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.