Anti-Vimentin Rabbit Polyclonal Antibody
| Cat Number: | ABS111308 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthetic peptidecorresponding to Mouse Vimentin |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1: 500-1: 1000 IHC/IF: 1: 1500-1: 3000 |
| Molecular: | 54 kDa |
| Purification: | Affinity purification |
| Synonyms: | VIM, CTRCT30, HEL113, vimentin |
| Background: | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
| Storage: | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
WB analysis of Vimentin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.
IHC analysis of Vimentin . Sample: Human breast cancer , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.
IHC analysis of Vimentin . Sample: Human breast cancer , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 1600, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.