AP-1
| Cat Number: | AB-84334 |
|---|---|
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human AP-1 around the non-phosphorylation site of T91. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 (diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute Plasma Membrane Isolation and Cell Fractionation Kit
Western blot analysis of lysates from HeLa cells using C-Jun antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Rat-brain
tissue. 1, AP-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-brain
tissue. 1, AP-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Negative control was used by secondary antibody only.
Immunofluorescence analysis of human-stomach
tissue. 1, AP-1 Polyclonal Antibody (red
was diluted at 1:200 (4°C, overnight). 2,
Cy3 labled Secondary antibody was diluted at 1:300
(room temperature, 50min).3,
Picture B: DAPI (blue) 10min.
Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B
Immunofluorescence analysis of human-stomach tissue.
1, AP-1 Polyclonal Antibody (red) was diluted at 1:200
(4°C, overnight). 2, Cy3 labled
Secondary antibody was diluted at 1:300
(room temperature, 50min).3,
Picture B: DAPI (blue) 10min.
Picture A:Target. Picture B: DAPI.
Picture C: merge of A+B