ARG1
| Cat Number: | AB-81437 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human ARG1 / Arginase 1 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 36kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ARG1;arginase-1 |
| Background: | Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using ARG1 /
Arginase 1 Antibody at
1:2000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.
Immunohistochemistry of paraffin embedded
mouse liver using ARG1 /
Arginase 1 antibody at
dilution of 1:100 (40x lens).
Immunofluorescence analysis of Rat
liver using ARG1 / Arginase 1 antibody
at dilution of 1:100. Blue:
DAPI for nuclear staining.
Immunofluorescence analysis of
Human liver using ARG1 / Arginase 1
antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse
liver using ARG1 / Arginase 1 antibody
at dilution of 1:100. Blue: DAPI
for nuclear staining.