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ARG1

Cat Number: AB-84248
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human ARG1 / Arginase 1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 36kDa
Purification: Aff. Pur.
Synonyms: ARG1;arginase-1
Background:

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84248-1.jpg

Western blot analysis of extracts of various cell lines, using ARG1 /Arginase 1 Antibody at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.

AB-84248-2.jpg

Immunohistochemistry of paraffin embedded
mouse liver using ARG1 / Arginase 1
antibody at dilution of 1:100 (40x lens).

AB-84248-3.jpg

Immunofluorescence analysis of Rat liver using ARG1 / Arginase 1 antibody at dilution of 1:100. Blue:
DAPI for nuclear staining.