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ATP6

Cat Number: AB-83828
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of mouse ATP6
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:50-1:100

Molecular: 20kDa
Purification: Aff. Pur.
Synonyms: MT-ATP6;ATPase6;MTATP6;ATP6
Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83828-1.jpg

Western blot analysis of extracts of various cell lines, using ATP6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)
(at 1:10000 dilution).
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit
Exposure time: 10s.

AB-83828-2.jpg

Immunofluorescence analysis of U2OS
cells using ATP6 antibody at dilution of
1:100. Blue: DAPI for nuclear staining.

References

References for ATP6 Rabbit pAb
Product:ATP6 Rabbit pAb
Journal:International Journal Of Biochemistry & Cell Biology
Application:WB
IF:5.085
Species:Sus scrofa
PMID:28583467
Title:Leucine reduces reactive oxygen species levels via an energy metabolism switch by activation of the mTOR-HIF-1α pathway in porcine intestinal epithelial cells

References for ATP6 Rabbit pAb
Product:ATP6 Rabbit pAb
Journal:Free Radical Biology and medicine
Application:WB
IF:7.376
Species:Homo sapiens
PMID:29704620
Title:Mitochondrial targeting of HIF-1α inhibits hypoxia-induced apoptosis independently of its transcriptional activity

References for ATP6 Rabbit pAb
Product:ATP6 Rabbit pAb
Journal:Redox biology
Application:WB
IF:11.799
Species:Mus musculus
PMID:30686776
Title:HIF-1α protects against oxidative stress by directly targeting mitochondria.

References for ATP6 Rabbit pAb
Product:ATP6 Rabbit pAb
Journal:Cell death & disease
Application:WB
IF:8.469
Species:Mus musculus
PMID:31235686
Title:Mitochondrial miR-762 regulates apoptosis and myocardial infarction by impairing ND2.

References for ATP6 Rabbit pAb
Product:ATP6 Rabbit pAb
Journal:Bioscience Reports
Application:WB
IF:3.84
Species:Rattus norvegicus
PMID:31778153
Title:HuoXue QianYang QuTan Recipe attenuates left ventricular hypertrophy in obese hypertensive rats by improving mitochondrial function through SIRT1/PGC-1α deacetylation pathway