ATPASE/Na+K+ alpha 1
| Cat Number: | AB-84189 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP1A1. |
| Reactivity: | Hu, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 115kDa |
| Purification: | Aff, Pur. |
| Synonyms: | ATP1A1transporting subunit alpha 1 |
| Background: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of A-549 cells, using ATPase Na+/K+ antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)
at 1:10000 dilution._Lysates/proteins:
25ug per lane._Blocking buffer: 3%
nonfat dry milk in TBST._Detection:
ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin embedded rat kidney using ATPase Na+/K+ antibody at dilution of 1:100
(40x lens).
Immunofluorescence analysis of U2OS cells using ATPase Na+/K+ antibody at dilution of 1:100. Blue: DAPI for nuclear staining.