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BCL-2

Cat Number: AB-83984
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 81-131 of human Bcl-2.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunofluorescence: 1:50 – 1:100
Immunoprecipitation: 1:50 – 1:100

Molecular: 27kDa
Purification: Aff. Pur.
Synonyms: Bcl-2;PPP1R50;BCL2
Background:

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83984-1.jpg

Western blot analysis of extracts of various cell lines,
using Bcl-2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
(H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

AB-83984-2.jpg

Immunofluorescence analysis of L929 cells
using Bcl-2 antibody at dilution of
1:100. Blue: DAPI for nuclear staining.

References

References for Bcl-2 Rabbit pAb
Product:Bcl-2 Rabbit pAb
Journal:Basic Research in Cardiology
Application:WB
IF:5.301
Species:Rattus norvegicus
PMID:28534118
Title:Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury

References for Bcl-2 Rabbit pAb
Product:Bcl-2 Rabbit pAb
Journal:Journal of Cardiovascular Translational Research
Application:WB
IF:2.319
Species:Rattus norvegicus
PMID:29404859
Title:Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2

References for Bcl-2 Rabbit pAb
Product:Bcl-2 Rabbit pAb
Journal:BMC medicine
Application:WB
IF:9.08
Species:Mus musculus
PMID:30782145
Title:Cathelicidin-related antimicrobial peptide protects against myocardial ischemia/reperfusion injury.

References for Bcl-2 Rabbit pAb
Product:Bcl-2 Rabbit pAb
Journal:Oncology Letters
Application:WB
IF:1.87
Species:Homo sapiens
PMID:31612057
Title:Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion

References for Bcl-2 Rabbit pAb
Product:Bcl-2 Rabbit pAb
Journal:Oncology letters
Application:WB
IF:1.87
Species:Homo sapiens
PMID:31612057
Title: Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion