Beclin 1 Rabbit Monoclonal Antibody
| Cat Number: | MABN21322 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG,Kappa |
| Immunogen: | Recombinant protein of human Beclin 1 |
| Reactivity: | Human,Mouse,Rat,Chicken |
| Applications: | WB 1:2000-1:10000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 |
| Molecular: | Observed MW:60kD |
| Purification: | Protein A |
| Synonyms: | Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197) |
| Background: | Cell localization:Cytoplasm.beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein |
| Storage: | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
Western blot analysis of lysates from Hela cells, using Beclin 1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, Beclin 1 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).