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GRP78 / BIP Rabbit Polyclonal Antibody

Cat Number: AB-E8358
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Synthetic peptide from human protein at AA range: 505-570.
Reactivity: Human,Mouse,Rat,Fish
Applications:

Western Blot: 1/500 – 1/2000

Immunohistochemistry(paraffin-embedded tissues):  1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: 78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)
Background:

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum  (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-E8358-1

Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

AB-E8358-2

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200

AB-E8358-3

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200

AB-E8358-4

Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L)  Alexa Fluor 488 Secondary antibody
was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.