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BMP2 Rabbit Monoclonal Antibody

Cat Number: MAB27101
Conjugate: Unconjugated
Size: 100 ug
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant protein.This information is considered to be commercially sensitive.
Reactivity: Human,Mouse,Rat
Applications: WB 1:1000 - 1:6000 IF/ICC 1:100 - 1:800 IP 0.5μg-4μg antibody for 300μg-500μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL.
Molecular: 12-45kDa
Purification: Affinity purification
Synonyms: BDA2; BMP2A; SSFSC; SSFSC1; BMP2
Background:

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients.
Form: liquid
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Western blot analysis of various lysates using BMP2 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Western blot analysis of various lysates using BMP2 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of various lysates using BMP2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Negative control (NC): LNCaP Exposure time: 1s.

Western blot analysis of various lysates using BMP2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Negative control (NC): LNCaP
Exposure time: 1s.

Confocal imaging of HeLa cells using BMP2 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with AF488-conjugated Goat Anti-Mouse IgG (H+L) Ab ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of HeLa cells using BMP2 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with AF488-conjugated Goat Anti-Mouse IgG (H+L) Ab ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.