Cat Number: AB-82904
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N-Cadherin
Reactivity: Hu, Ms, Rt

Western Blot, WB (1: 500- 1:1,000)
Immunohistochemistry, IHC-P (1:10- 1:100)

Purification: Purified
Synonyms: CDH2; CD325; CDHN; CDw325; NCAD; cadherin-2

This gene encodes a classical cadherin and member of the cadherin superfamily.Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

Form: Liquid
Storage: 2-8°C for short term for longer term at -20°C for one year.Avoid repeated freeze and thaw cycles.

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.


Immunohistochemistry of paraffin-embedded mouse liver using N-Cadherin Antibody at dilution of 1:100