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Cadherin-N

Cat Number: MAB-94622
Conjugate: Unconjugated
Size: 100 ug
Clone: 5D5
Concentration: 1mg/ml
Host: MS
Isotype: IgG1
Immunogen: Purified recombinant fragment of human N-cadherin expressed in E. Coli.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Immunofluorescence: 1:200-1:1000
Flow Cytometry: 1:200-1:400
ELISA: 1:10000

Molecular: 100kDa
Purification: Aff. Pur.
Background:

Specificity: N-cadherin monoclonal antibody detects endogenous levels of N-cadherin protein.

Form: Liquid
Buffer: Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Storage: Store at-20°C, and avoid repeat freeze-thaw cycles.
MAB-94622-1.jpg

Western blot analysis using N-cadherin monoclonal antibody against A431 (1) , NIH/3T3 (2) , HeLa (3) , C6 (4) and LNCap (5) cell lysate.

MAB-94622-2.jpg

Immunohistochemistry analysis of paraffin-embedded human
lung cancer (A) , colon cancer (B) , ovarian cancer (C) and
mammary cancer (D) with DAB staining using N-cadherin
monoclonal antibody.

MAB-94622-3.jpg

Immunofluorescence analysis of A431 cells using N-cadherin monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

MAB-94622-4.jpg

Flow cytometric analysis of PC-2 cells using N-cadherin
monoclonal antibody (green) and negative control (purple).