Calnexin Rabbit Polyclonal Antibody
| Cat Number: | ABS111369 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Calnexin |
| Reactivity: | Species, Human, Mouse, Rat, Human, Mouse |
| Applications: | Applications Dilution WB 1: 500-1: 1000 ICC/IF 1: 50-1: 100 |
| Purification: | Affinity purification |
| Synonyms: | Canx, CALX, CNX, FLJ26570, IP90, p90, Major histocompatibility complex class I antigen-binding protein p88, CANX |
| Background: | Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of Calnexin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
WB analysis of Calnexin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
ICC analysis of Calnexinred.Sample: HeLa, 4% PFA 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 50, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.