|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 18-417 of human CALR (NP_004334.1).|
|Reactivity:||Hu, Ms, Rt|
Western Blot: 1:500 – 1:1000
|Synonyms:||CALR; CRT; HEL-S-99n; RO; SSA; cC1qR; calreticulin|
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of
various cell lines, using CALR antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
Detection: ecl WEST Pico Plus
Exposure time: 1s.
Immunofluorescence analysis of HeLa
cells using CALR antibody. Blue:
DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using CALR antibody.
at dilution of 1:400. Blue: DAPI for nuclear
Confocal immunofluorescence analysis of
Hela cells using CALR at
dilution of 1:400. Blue: DAPI for nuclear
Immunoprecipitation analysis of 200ug
extracts of HepG2 cells using 1ug CALR
antibody. Western blot was
performed from the immunoprecipitate
using CALR antibody at a dilition