Caspase 3 (cleaved) ASP175 Rabbit Polyclonal Antibody
| Cat Number: | AB-83787 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:126-175 |
| Reactivity: | Human;Mouse;Rat |
| Applications: | Western blotting 1:1000 |
| Molecular Weight: | 17,35kDa |
| Purification: | Polyclonal antibodies are producedby immunizing animals with a synthetic peptide correspondingto amino-terminal residues adjacent to (Asp175) inhuman caspase-3. |
| Synonyms: | CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1 |
| Background: | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is one of the key executioners of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADPribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases. This antibody detects non-specific caspase substrates by western blot. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Nuclear background may be observed in rat and monkey samples. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C, and avoid repeat freezethawcycles |
Western blot analysis of extracts using Caspase-3 Antibody(upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).Postsurgical wound management and prevention of triple-negative breast cancer recurrence with a pryoptosis-inducing, photopolymerizable hydrogel.
Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.
Immunohistochemical analysis of rat kidney tissue.
The antibody was diluted at 1:200 (4°C,overnight
Sodium citrate pH6.0 was used for antibody retrieval
(>98°C, 20min). Secondary antibody was diluted at
1:200 (room temperature, 30min).
Immunohistochemical analysis of mouse colon tissue.
The antibody was diluted at 1:200 (4°C,overnight).
Sodium citrate pH6.0 was used for antibody retrieval
(>98°C, 20min). Secondary antibody was diluted
at 1:200 (room temperature, 30min)
Immunohistochemical analysis of human liver
tissue. Anti-Cleaved-Caspase-3 p17 (D175) at
1:200 (4°C,overnight). Antigen retrieval – Sodium
Citrate pH6 (>98°C, 20min). Secondary – 1:200
(room temp, 30min). Negative control –
Secondary only