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Caspase 3 (cleaved)

Cat Number: AB-83773
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human Caspase-3 p17
Reactivity: HU, Ms,Rt
Applications:

Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin-embedded tissues): 1:100-1:300
Immunofluorescence: 1:200
ELISA: 1:20000

Molecular: 31/17kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
Background:

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADPribose) polymerase (PARP) at a ‘216-Asp-|-Gly-217’ bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.Caspase-3 p17 is a protein encoded by the CASP3 gene which is approximately 31,6 kDa. Caspase-3 p17 is localised to the cytoplasm. It is involved in the TNFR1 pathway, apoptosis modulation and signalling and respiratory electron transport. This protein falls under the cysteine-aspartic acid protease family. It is a sequential activatior of caspases and plays a central role in the execution-phase of cell apoptosis. Caspase-3 p17 is highly expressed in lung, spleen, heart, liver and kidney. Mutations in the CASP3 gene may result in bladder urothelial carcinoma and primary effusion lymphoma. AB-83773 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment activated Caspase-3 p17 protein resulting from cleavage adjacent to ASP175. Specificity: Cleaved-Caspase-3 p17 (ASP175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to ASP175. Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83773-1.jpg

Western blot (WB) analysis of Cleaved-Caspase-3 p17 (ASP175) Polyclonal Antibody.

AB-83773-2.jpg

Immunohistochemical analysis of human-colon tissue. Anti-Cleaved-Caspase-3 p17 (ASP175) at 1:200 (4°C,overnight). Antigen retrieval – Sodium Citrate pH6 (>98°C, 20min). Secondary – 1:200 (room temp, 30min). Negative control – Secondary only

AB-83773-3.jpg

Immunofluorescence analysis of Human lung cancer tissue. 1: Cleaved-Caspase-3 p17 (ASP175) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight). 2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.

AB-83773-4.jpg

Immunofluorescence analysis of Human lung cancer tissue. 1: Cleaved-Caspase-3 p17 (ASP175) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight). 2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.