Caspase 8
| Cat Number: | AB-82858 |
|---|---|
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human Caspase-8. |
| Reactivity: | Ms, Rt, Hu |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 17kDa/44kDa/60kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ALPS2B;CAP4;Casp-8;FLICE;MACH;MCH5;Caspase 8;CASP8;Caspase-8;caspase-8 |
| Background: | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4 |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of extracts of various cell lines, using Caspase-8 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunohistochemistry of paraffin embedded human colon carcinoma using
Caspase-8 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin embedded mouse kidney using Caspase-8
antibody at dilution of 1:200 (40x
lens).
Immunofluorescence analysis of NIH/3T3 cells using Caspase-8 antibody at dilution of 1:100. Blue: DAPI for nuclear
staining.
Review for Caspase-8 Rabbit Polyclonal Antibody
Experiment Type Western blot(WB)
Sample N9
Description: Western blot analysis of extracts of N9 cells, using antibody at 1:200 dilution.
Review for Caspase-8 Rabbit Polyclonal Antibody
References
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:EMBO Molecular Medicine
Application:WB
IF:9.237
Species:Mus musculus
PMID:29335338
Title:CRTH2 promotes endoplasmic reticulum stress‐induced cardiomyocyte apoptosis through m‐calpain
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Frontiers in microbiology
Application:WB
IF:
Species:Bos taurus
PMID:29670605
Title:Antiviral Immunotoxin Against Bovine herpesvirus-1: Targeted Inhibition of Viral Replication and Apoptosis of Infected Cell
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Oncology reports
Application:WB
IF:3.04
Species:Homo sapiens
PMID:31115582
Title:Cytokeratin 18 regulates the transcription and alternative splicing of apoptotic related genes and pathways in HeLa cells.
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Molecular Neurodegeneration
Application:WB
IF:8.27
Species:Mus musculus
PMID:32295623
Title:NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Experimental cell research
Application:WB
IF:3.32
Species:Mus musculus
PMID:31202710
Title:The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Redox Biology
Application:WB
IF:7.79 Species:
Mus musculus
PMID:31926620
Title:Triggering apoptosis by oroxylin A through caspase-8 activation and p62/SQSTM1 proteolysis
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Investigational new drugs
Application:WB
IF:3.5
Species:Homo sapiens,Mus musculus
PMID:30924024
Title:Emodin induced necroptosis in the glioma cell line U251 via the TNF-α/RIP1/RIP3 pathway.
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Cell death & disease
Application:WB
IF:5.95
Species:Rattus norvegicus
PMID:31221990
Title:Microglia-derived TNF-α mediates endothelial necroptosis aggravating blood brain-barrier disruption after ischemic stroke.
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Free radical biology & medicine
Application:WB
IF:5.65
Species:Homo sapiens,Mus musculus
PMID:31560953
Title:L-V9 exerts anti-T cell malignancies effects via promoting lysosome-dependent AKT1 degradation and activating AKT1/FOXO3A/BIM axis.
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Frontiers in Pharmacology
Application:B
IF:3.845
Species:Mus musculus
PMID:29441017
Title:15, 16-Dihydrotanshinone I Inhibits Hemangiomas through Inducing Pro-apoptotic and Anti-angiogenic Mechanisms in Vitro and in Vivo.
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:International Journal of Molecular Sciences
Application:WB
IF:4.183
Species:Homo sapiens
PMID:31569691
Title:Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway
References for Caspase-8 Rabbit pAb
Product:Caspase-8 Rabbit pAb
Journal:Carcinogenesis
Application:WB
IF:4
Species:Homo sapiens
PMID:31306481
Title: Histone Methyltransferase SETDB1 Promotes Colorectal Cancer Proliferation through the STAT1-CCND1/CDK6 Axis.