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Caspase 9

Cat Number: AB-82859
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human Caspase-9
Reactivity: Ms, Rt, Hu
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:100

Molecular: 36kDa/46kDa
Purification: Aff. Pur.
Synonyms: CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
Background:

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Form: Liquid
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
AB-82859-1.jpg

Western blot analysis of extracts of various cell lines, using Caspase-9 antibody at 1:300
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

AB-82859-2.jpg

Western blot analysis of extracts of Jurkat cells, using Caspase-9 antibody at 1:1000
dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.

AB-82859-3.jpg

Immunohistochemistry of paraffin embedded
rat heart using Caspase-9 antibody at dilution of 1:100 (40x lens).

AB-82859-4.jpg

Caspase-9 Rabbit pAb5

Experiment Type Western blot(WB)

References