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Catenin beta-1/CTNNB1

Cat Number: MAB-94660
Size: 100 ug
Clone: CB22
Concentration: 1mg/ml
Host: Ms
Isotype: IgG
Immunogen: A synthesized peptide derived from human beta Catenin.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200
Immunoprecipitation: 1:50 – 1:200
Molecular Weight: 92kDa
Purification: Aff. Pur.
Synonyms: CTNNB;MRD19;armadillo;beta Catenin;CTNNB1
Background:

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Form: Liquid
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-94660-1.jpg

Western blot analysis of extracts of HeLa cells, using β-Catenin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. Exposure time: 10s.

MAB-94660-2.jpg

Western blot analysis of extracts of Mouse brain, using β-Catenin antibody at 1:1000
dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. Exposure time: 10s.

MAB-94660-3.jpg

Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using
β-Catenin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

MAB-94660-4.jpg

Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug β-Catenin antibody. Western blot was performed from the immunoprecipitate using β-Catenin at a dilition of 1:1000.

MAB-94660-5.jpg

Immunohistochemistry of paraffin embedded
Human cervical squamous cell
carcinoma using [KO Validated] β-Catenin
Rabbit mAb at dilution of 1:100 (40x lens).

MAB-94660-6.jpg

Immunofluorescence analysis of C6 cells using β-Catenin antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.

References

References for [KO Validated] β-Catenin Rabbit mAb
Product:[KO Validated] β-Catenin Rabbit mAb
Journal:Evid Based Complement Alternat Med
Application:WB
IF:1.81
Species:Homo sapiens
PMID:33014112
Title:Role of Chrysophanol in Epithelial-Mesenchymal Transition in Oral Cancer Cell Lines via a Wnt-3-Dependent Pathway

References for [KO Validated] β-Catenin Rabbit mAb
Product:[KO Validated] β-Catenin Rabbit mAb
Journal:Stem Cell Res Ther
Application:WB
IF:5.08
Species:Rattus norvegicus
PMID:33712069
Title:Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat model.

References for [KO Validated] β-Catenin Rabbit mAb
Product:[KO Validated] β-Catenin Rabbit mAb
Journal:Front Pharmacol
Application:WB
IF:
Species: Homo sapiens
PMID: 33708105
Title: Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells