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Cathepsin V Rabbit Polyclonal Antibody

Cat Number: AB-84813
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 105-334 of human CTSV
Reactivity: Human,Mouse,Rat
Applications:

Western Blot:   1:500 – 1:2000

Molecular Weight: 37kDa
Purification: Affinity purification
Synonyms: CTSU; CATL2; CTSL2; CTSV
Background:

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or
peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84812-1

Western blot analysis of extracts of various cell lines, using CTSV antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus
Exposure time: 30s.