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Caveolin 1 (CAV1)

Cat Number: AB-81830
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Caveolin-1.
Reactivity: Hu, Ms
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 20kDa/23kDa
Purification: Aff. Pur.
Synonyms: BSCL3;CGL3;LCCNS;MSTP085;PPH3;VIP21;CAV1
Background:

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-81830-1.jpg

Western blot analysis of extracts of various cell lines, using Caveolin-1 antibody at 1:3000
dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-81830-2.jpg

Western blot analysis of extracts of C6 cells, using Caveolin-1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-81830-3.jpg

Immunohistochemistry of paraffin embedded rat spleen using Caveolin-1 Rabbit pAb at dilution of 1:100 (40x lens).

AB-81830-4.jpg

Immunohistochemistry of paraffin embedded human liver using Caveolin-1
Rabbit pAb dilution of 1:100 (40x lens).

AB-81830-5.jpg

Immunofluorescence analysis of Mouse
lung cells using Caveolin-1 antibody
at dilution of 1:100.
Blue: DAPI for nuclear staining.

AB-81830-6.jpg

Immunofluorescence analysis of Rat lung
cells using Caveolin-1 antibody at
dilution of 1:100.
Blue: DAPI for nuclear staining.

References

References for Caveolin-1 Rabbit pAb
Product: Caveolin-1 Rabbit pAb
Journal: Toxicology and applied pharmacology
Application: WB
IF:
Species: Homo sapiens
PMID: 31381904
Title: The involvement of lipid raft pathway in suppression of TGFβ-mediated metastasis by tolfenamic acid in hepatocellular carcinoma cells.

References for Caveolin-1 Rabbit pAb
Product: Caveolin-1 Rabbit pAb
Journal: PLoS Pathog
Application: WB
IF:6.21
Species:Homo sapiens
PMID:33315947
Title:Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45