CCL2 Mouse Monoclonal Antibody
| Cat Number: | MABN81118 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | Mouse IgG1 |
| Immunogen: | Purified recombinant fragment of human CCL2 expressed in E. Coli. |
| Reactivity: | Human,Mouse,Rat,Monkey |
| Applications: | WB 1:500-1:2000,IHC 1:200-1:1000,ICC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400 |
| Molecular: | 11kDa |
| Purification: | Affinity Purification |
| Synonyms: | HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30 |
| Background: | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. |
| Form: | Liquid |
| Buffer: | Purified antibody in PBS with 0.05% sodium azide |
| Storage: | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using CCL2 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6)cell lysate.
Immunofluorescence analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.