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CD28

Cat Number: AB-84284
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Gt
Isotype: IgG
Immunogen: internal region
Reactivity: Hu
Applications:

WB, IHC, ELISA

Molecular Weight: 25.1 kDa
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms: T-cell-specific surface glycoprotein CD28TP44CD antigen CD28
Background:

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival, Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.

Form: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20¬¨¬®‚ÄöÖ†ÖªC. Minimize freezing and thawing.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
AB-84284-1.jpg

(0.3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

AB-84284-2.jpg

Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

AB-84284-3.jpg

2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.