CD3 epsilon (activation epitope) Mouse Monoclonal Antibody

Cat Number: MAB-94775
Conjugate: Unconjugated
Size: 100 ug
Clone: APA1/1
Concentration: 1mg/ml
Host: Mouse
Isotype: IgG1
Immunogen: Purified human CD3 proteins isolated from thymus
Reactivity: Mouse, Human

Flow cytometry: 1-4 μg/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/ml).

Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.
Western blot
Immunohistochemistry (frozen tissues)

Purification: Purified by protein-A affinity chromatography.
Synonyms: CD3 epsilon chain, T3E, IMD18

Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS.

Form: Liquid
Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage: Store at 2-8°C. Do not freeze.