CD3 Rabbit Monoclonal Antibody
| Cat Number: | MABN21534 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG,Kappa |
| Immunogen: | A synthetic peptide of human CD3 epsilon |
| Reactivity: | Human,Mouse,Rat,Monkey |
| Applications: | IHC1:200-1:1000;WB1:1000-1:5000;IF1:200-1:1000;ELISA1:5000-1:20000 |
| Molecular: | Calculated MW:23kD;Observed MW:23kD |
| Purification: | Protein A |
| Synonyms: | T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD antigen CD3e |
| Background: | Cell localization:Membranous.The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma,-deltaand-zeta,andtheT-cellreceptoralpha/betaandgamma/delta heterodimers,formstheT-cellreceptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.The genes encodingthe epsilon, gammaanddelta polypeptidesare locatedinthesame clusteron chromosome 11.TheepsilonpolypeptideplaysanessentialroleinT-celldevelopment.Defectsinthisgenecauseimmunodeficiency.This genehasalsobeenlinkedtoasusceptibilitytotypeIdiabetesinwomen. |
| Form: | Liquid |
| Buffer: | PBS,50%glycerol,0.05%Proclin300,0.05%protective protein |
| Storage: | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
Western blot analysis of lysates from Jurkat cells, using CD3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue. 1, CD3 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature,30min).
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue. 1, CD3 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature,30min).