CD31 Rabbit Polyclonal Antibody
| Cat Number: | ABN03853 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μL |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PECAM-1. AA range:686-735 |
| Reactivity: | Human,Mouse |
| Applications: | WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000 |
| Molecular: | 83 kDa |
| Purification: | Affinity purification |
| Synonyms: | Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31 |
| Background: | PECAM-1 a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% sodium azide, pH 7.3. |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Western blot analysis of CD31 in HepG2, K562, L929, mouse brain lysates using CD31 antibody.
Immunofluorescence analysis of CD31 in HeLa using CD31 antibody. The picture on the right is blocked using synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded CD31 in paraffin-embedded Human kidney using CD31 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.