CD324 / E-cadherin Mouse Monoclonal Antibody

Cat Number: MAB-93240
Conjugate: Unconjugated
Size: 100ug
Clone: 67A4
Concentration: 1mg/ml
Host: Mouse
Isotype: IgG1
Immunogen: T-47D cells
Reactivity: Human

Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry(paraffin-embedded tissues), Flow Cytometry

Working dilutions: Immunohistochemistry (frozen sections):4-8 μg/ml
Flow cytometry:5-10 μg/ml

Purification: Purified by protein-A affinity chromatography.
Synonyms: Cadherin-1, E-cadherin, Uvomorulin, CAM 120/80, UVO, CDHE, ECAD, LCAM, BCDS1, Arc-1

The mouse monoclonal antibody 67A4 recognizes an extracellular epitope of CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.

Form: Liquid
Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide.
Storage: Store at 2-8°C. Do not freeze.

Western blot analysis of extracts of HCT116 cells, using CDH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.