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CD366 / TIM-3/ (HAVCR2)

Cat Number: AB-84287
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-202 of human TIM-3/HAVCR2
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry: 1:50 – 1:200
Molecular Weight: 33kDa
Purification: Aff. Pur.
Synonyms: HAVCR2;CD366;HAVcr-2;KIM-3;TIM3;TIMD-3;TIMD3;T im-3
Background:

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance
Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
AB-84287-1.jpg

Western blot analysis of extracts of
various cell lines, using
TIM-3/HAVCR2 antibody at
1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL Bas
Exposure time: 10s.

AB-84287-2.jpg

Western blot analysis of extracts of
various cell lines, using
TIM-3/HAVCR2 antibody at
1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.

AB-84287-3.jpg

Immunohistochemistry of
Paraffin embedded
human tonsil using
TIM-3/HAVCR2 antibody
At dilution of 1:200 (40x lens).