|Immunogen:||A synthetic peptide corresponding of human CD56 different from the related Human sequence by single amino acid.|
|Reactivity:||Hu, Ms, Rt|
Western blot: 1:500 – 1:2000
NCAM is a membrane-bound glycoprotein that plays a role in cell-cell and cell-matrix adhesion through both its homophilic and heterophilic binding activity. The neural cell adhesion molecule appears on early embryonic cells and is important in the formation of cell collectives and their boundaries at sites of morphogenesis. Later in development it is found on various differentiated tissues and is a major CAM mediating adhesion among neurons and between neurons and muscle. NCAM gene is mapped to 11q23. The neural cell adhesion molecule (NCAM) can influence a number of diverse intercellular events, including junctional communication, the association of axons with pathways and targets, and signals that alter levels of neurotransmitter enzymes.
|Buffer:||Liquid in 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4.|
|Storage:||At + 4°C for short term storage and at -20°C for longer time. Avoid Freeze and Thaw cycles.|
Western blot analysis of extracts of various cell lines, using NCAM1/CD56 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Pico Kit
Exposure time: 90s
Immunohistochemistry of paraffin-embedded human stomach using NCAM1 / CD56 antibody at dilution of 1:100
Immunofluorescence analysis of C6 cells using NCAM1 / CD56 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.