CD8 α Rabbit Monoclonal Antibody
| Cat Number: | MABN21584 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1 mg/ml. The concentration of this product may be batch-dependent |
| Host: | Rabbit |
| Isotype: | IgG,Kappa |
| Immunogen: | A synthetic peptide of human CD8alpha |
| Reactivity: | Human,Mouse,Rat |
| Applications: | IHC1:200-1000;WB1:1000-5000;IF1:200-1000;ELISA1:5000-20000 |
| Molecular: | Observed MW:35kD |
| Purification: | Protein A |
| Synonyms: | T-cell surface glycoprotein CD8 alpha chain;T-lymphocyte differentiation antigen T8/Leu-2;CD antigen CD8a |
| Background: | Cell localization:Membranous.The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.This gene encodes the CD8 alpha chain. Multiple transcript variants encoding differential isoforms have been found for this gene.[provided by RefSeq,Nov 2011] |
| Form: | Liquid |
| Buffer: | PBS,50% glycerol,0.05% Proclin300,0.05% protective protein |
| Storage: | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles |
WesternblotanalysisoflysatesfromMousethymuscells, using CD8 α Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibodywasusedtodetecttheantibody.
Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, CD8 α Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature,30min).
Species:HumanSite:tonsil;Sample: Paraffin-embedded section; Antibody concentration: 1/1,000