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CD9

Cat Number: AB-83880
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinan protein of Human CD9
Reactivity: Hu, Ms, Rt
Applications:

Western Blot : 1:1000
Immunohistochemistry: 1:50 – 1:100
Immunofluorescence: 1:50 – 1:100
Immunoprecipitation: 1:20 – 1:50

Molecular Weight: 25kDa
Purification: Aff. Pur.
Synonyms: CD9; BTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29; CD9 antigen
Background:

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays acritical role in the suppression of cancer cell motility and metastasis.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage: At +4°C for short term. At -20°C for longer term. Avoid freeze / thaw cycles.
AB-83880-1.jpg

Western blot analysis of extracts of various cell line, using CD9 antibody
at a dilution of 1:1000.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

AB-83880-2.jpg

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue,using CD9 antibody.
The picture on the right is blocked with the synthetized peptide.

AB-83880-3.jpg

Immunofluorescence analysis of HeLa cells using CD9 antibody.
The picture on the right is blocked with the synthetized peptide.