Top

Cleaved-Caspase-9 p35 (D315) Rabbit Polyclonal Antibody

Cat Number: AB-E5700
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:266-315
Reactivity: Human;Rat;Mouse;
Applications:

Western BLot:   1:500-2000

Immunohistochemistry (paraffin-embedded tissues):    1:50-300

Immunofluorescence:  1:50-300
Molecular Weight: 35 -46kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
Background:

CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step
is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium az
Storage: Store at -20℃. Avoid repeated freeze-thaw cycles.
AB-E5700-1

Western Blot analysis of 293 cells using Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody diluted at 1:1000

AB-E5700-2

Western Blot analysis of various cells using Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody diluted at 1:1000

AB-E5700-3

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp315) Antibody. The picture on the right is blocked with the synthesized peptide.

AB-E5700-4

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

AB-E5700-5

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

AB-E5700-6

Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

AB-E5700-7

Immunofluorescence analysis of Human-breast tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B