Cleaved-Notch 1 (V1754) Rabbit Polyclonal Antibody
| Cat Number: | AB-84808 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784 |
| Reactivity: | Human;Mouse;Rat |
| Applications: | Western Blot: 1:500-2000 |
| Molecular Weight: | 110kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1 |
| Background: | notch 1(NOTCH1) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymph. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of various cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1:500
Western Blot analysis of KB cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1:500
Qu, Xiaochen, et al. “MiR‐199b‐5p inhibits osteogenic differentiation in ligamentum flavum cells by targeting JAG1 and modulating the Notch signalling pathway.” Journal of cellular and molecular medicine 21.6 (2017): 1159-1170.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Notch 1 (Cleaved-Val1754) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Hela cell. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Immunofluorescence analysis of Human-lung-cancer tissue. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B